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KMID : 0545120170270010043
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 1 p.43 ~ p.48
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Antimicrobial Activity of the Scolopendrasin V Peptide Identified from the Centipede Scolopendra subspinipes mutilans
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Lee Joon-Ha
Kim In-Woo
Kim Mi-Ae
Ahn Mi-Young
Yun Eun-Young
Hwang Jae-Sam
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Abstract
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In a previous study, we analyzed the transcriptome of Scolopendra subspinipes mutilans using next-generation sequencing technology and identified several antimicrobial peptide candidates. One of the peptides, scolopendrasin V, was selected based on the physicochemical properties of antimicrobial peptides using a bioinformatics strategy. In this study, we assessed the antimicrobial activities of scolopendrasin V using the radial diffusion assay and colony count assay. We also investigated the mode of action of scolopendrasin V using flow cytometry. We found that scolopendrasin V’s mechanism of action involved binding to the surface of microorganisms via a specific interaction with lipopolysaccharides, lipoteichoic acid, and peptidoglycans, which are components of the bacterial membrane. These results provide a basis for developing peptide antibiotics.
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KEYWORD
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Antimicrobial peptide, membrane damage, bacterial membrane, scolopendrasin V, Scolopendra subspinipes mutilans
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